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The antibody is from purified rabbit serum by affinity purification by means of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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Overexpression of the gene has been noticed in lots of tumors, which results in chromosome instability, and so may well lead to tumorigenesis. This protein was discovered to affiliate with, and become associated with, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in mobile-cycle regulated histone gene expression and performs a essential position in marketing cell-cycle progression inside the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinct isoforms, are actually described. Two additional splice variants ended up noted but detailed nucleotide sequence facts just isn't yet readily available.

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Blocking peptides are peptides that bind precisely for the focus on antibody and block antibody binding. These peptide usually is made up of the epitope regarded from the antibody.

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This guide outlines further more doable optimization of cellular and immunoassay parameters to make sure the very best effects are...

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This guideline outlines further doable optimization of cellular and immunoassay parameters to make sure the absolute best final results are...

Antibodies bound to the blocking peptide now not bind towards the epitope on the target protein. By evaluating the staining within the blocked antibody versus the antibody by itself, you can see which staining is specific.

Target Details Cyclin E belongs on the remarkably conserved cyclin spouse and children, whose members are characterized by a remarkable periodicity in protein abundance with the cell cycle. Cyclins perform as regulators of CDK kinases. Unique cyclins show unique expression and degradation designs which add on the temporal coordination of each mitotic celebration. Cyclin E kinds a fancy with and features being a regulatory subunit of CDK2, whose activity is needed for mobile cycle G1/S changeover. This protein accumulates on the G1-S stage boundary and is also degraded as cells progress by means of S phase.

The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by both equally antibodies...

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